Museo Zacatecan, or Zacatecan Museum, is located in the city's historic center and was the headquarters of the Zacatecas Mint, which was founded in 1826. Federico Sescosse Lejeune, a Zacatecan celebrity who dedicated his life to defending and maintaining the cultural legacy of Zacatecas, founded the museum in 1995. Its collection is divided into three thematic lines: Huichol Culture and Art, an encounter with a living ancestral culture that is part of today's cultural diversity in Mexico; Popular Religious Imaginary of the Nineteenth Century, an approach to popular religious iconography that is a relic of an artistic past; and Colonial Forged Irons, notable examples of applied arts.

The primary temple of the Diocese of Zacatecas is the Zacatecas Cathedral, which is dedicated to the Virgin of the Assumption. Located in the city's historic core and is designated by UNESCO as a Cultural Heritage of Humanity. There were two temples before the current structure. The earliest was constructed in 1568 as a tiny parish of the city. In the place of the old temple, a second temple was erected, which was consecrated in 1625.

The Manuel Felguérez Abstract Art Museum was founded by a visionary and philanthropist. The Museum of Abstract Art, housed in the old Pursima Concepción Seminary (19th century) and later a prison for part of the 20th century, has among its collections a large anthological exhibition of Manuel Felguérez, as well as an important collective collection of artists' Abstracts ranging from the Generation of La Rupture (the 1950s) to the present day, providing an important testimony of the development of this plastic current in Mexico. The museum has 15 permanent rooms featuring paintings and sculptures by Mexican and foreign abstract artists, as well as temporary, solo, and group exhibits by artists with distinguished careers.

The Museo Rafael Coronel, housed in the ancient San Francisco Convent, houses an impressive collection of Mexican folk art, centered on the primary exhibit "The Face of Mexico," which includes over 5,000 Mexican masks. This museum also displays a portion of the Rosette Aranda Company's colorful puppet collection, an intriguing exhibition of pre-Columbian pots and vases, various drawings, sketches, and architectural projects by Diego Rivera, terracotta figurines from colonial Mexico, and other pieces from various pre-Hispanic, colonial, and contemporary times.